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Table 1 Theoretical and measured compositions of T. reesei secretome

From: A new stoichiometric miniaturization strategy for screening of industrial microbial strains: application to cellulase hyper-producing Trichoderma reesei strains

  theoretical measured
  mass content mass content molar composition
C 53.0 ± 0.9% 47.3 ± 0.1% 1
H 6.6 ± 0.1% 6.83 ± 0.04% 1.733
O 23.2 ± 0.7% 32.0 ± 0.8% 0.508
N 16.7 ± 0.2% 12.7 ± 0.5% 0.23
S 1.6 ± 0.5% 1.00 ± 0.05% 0.008
Total 100.1 ± 2.4% 99.7 ± 0.6%  
  1. Three independent final secretomes produced by T. reesei CL847 iβ under reference fed-batch production in bioreactor (with lactose feed) were desalted and analysed for their elementary compositions (values are mean and standard deviation of the 3 secretomes). Theoretical composition was based on amino-acids sequences of the fourth main cellulases.