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Table 1 Theoretical and measured compositions of T. reesei secretome

From: A new stoichiometric miniaturization strategy for screening of industrial microbial strains: application to cellulase hyper-producing Trichoderma reesei strains

 

theoretical

measured

 

mass content

mass content

molar composition

C

53.0 ± 0.9%

47.3 ± 0.1%

1

H

6.6 ± 0.1%

6.83 ± 0.04%

1.733

O

23.2 ± 0.7%

32.0 ± 0.8%

0.508

N

16.7 ± 0.2%

12.7 ± 0.5%

0.23

S

1.6 ± 0.5%

1.00 ± 0.05%

0.008

Total

100.1 ± 2.4%

99.7 ± 0.6%

 
  1. Three independent final secretomes produced by T. reesei CL847 iβ under reference fed-batch production in bioreactor (with lactose feed) were desalted and analysed for their elementary compositions (values are mean and standard deviation of the 3 secretomes). Theoretical composition was based on amino-acids sequences of the fourth main cellulases.
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Microbial Cell Factories

ISSN: 1475-2859

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