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Figure 6 | Microbial Cell Factories

Figure 6

From: A new stoichiometric miniaturization strategy for screening of industrial microbial strains: application to cellulase hyper-producing Trichoderma reesei strains

Figure 6

Application of the miniaturized fed-flask protocol to clone screening. Reference strain T. reesei CL847 iβ (black diamonds), and five recombinant strains: negative control E (gray diamonds) and clones A,B,C,D (respectively in blue squares, red cross, green triangles, orange circles), were cultivated in 50 mL shake flasks following fed-flask protocol: growth phase on 15 g L-1 glucose for 50 h then fed-batch with mixed lactose and ammonia feed (see Table 2). pH (A), biomass (B) and protein (C) monitoring, and specific protein production rate (D). Specific protein production rates were calculated over production phase (at constant biomass) and are mean and standard deviation of two independent replicates

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Microbial Cell Factories

ISSN: 1475-2859

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