Figure 4

Validation of the miniaturized fed-flask protocol design. Reference strain T. reesei CL847 iβ was cultivated in 50 mL shake-flask following shake flask pulse protocol (green triangles), or fed-flask protocol buffered with 25 (blue diamonds) or 50mM (red squares). pH (A), biomass concentration (B) and protein concentration (C) monitoring, and specific protein production rate calculation (moving average) (D)