Figure 4From: A new stoichiometric miniaturization strategy for screening of industrial microbial strains: application to cellulase hyper-producing Trichoderma reesei strainsValidation of the miniaturized fed-flask protocol design. Reference strain T. reesei CL847 iβ was cultivated in 50 mL shake-flask following shake flask pulse protocol (green triangles), or fed-flask protocol buffered with 25 (blue diamonds) or 50mM (red squares). pH (A), biomass concentration (B) and protein concentration (C) monitoring, and specific protein production rate calculation (moving average) (D)Back to article page