Microbial Cell Factories

Table 2 Yields of o-chloroacetophenone reductions catalyzed by E. coli whole-cell biocatalysts variable in XR and FDH activities.

From: Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

E coli	pH	XR (U/g_CDW)	FDH (U/g_CDW)	Substrate (mM)	Yield (mM)¹	Yield (mM)^{1, 2}
BL21_XR_FDH	7.5	1406	110	100	19	46
Rosetta_XR_2FDH	7.5	1140	251	100	35	67
BL21_XR_FDH	6.2	2226	48	100	27	78
Rosetta_XR_2FDH	6.2	2024	189	100	63	95

¹Determined for a reaction time of 8 h. ²Additives: 36 μM Polymyxin B sulphate and 500 μM NAD⁺.

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ISSN: 1475-2859

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