Skip to main content

Advertisement

Table 2 Yields of o-chloroacetophenone reductions catalyzed by E. coli whole-cell biocatalysts variable in XR and FDH activities.

From: Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

E coli pH XR (U/gCDW) FDH (U/gCDW) Substrate (mM) Yield (mM)1 Yield (mM)1, 2
BL21_XR_FDH 7.5 1406 110 100 19 46
Rosetta_XR_2FDH 7.5 1140 251 100 35 67
BL21_XR_FDH 6.2 2226 48 100 27 78
Rosetta_XR_2FDH 6.2 2024 189 100 63 95
  1. 1Determined for a reaction time of 8 h. 2Additives: 36 μM Polymyxin B sulphate and 500 μM NAD+.