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Table 1 FDH activities measured in the crude cell extracts of single- and co-expression strains at two induction temperatures.

From: Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

E coli strain

Cb FDH (U/gCDW) at 25°C

Cb FDH (U/gCDW) at 18°C

JM109_FDH

408 (reference, [8])

n.d.

BL21_FDH

402 ± 381

450

Star_FDH

458

551

Rosetta_FDH

445

614

BL21_XR_FDH

85 ± 122

110 ± 202

BL21_XR_2FDH

146 ± 301

186 ± 302

Rosetta_XR_FDH

100 ± 181

138 ± 232

Rosetta_XR_2FDH

202 ± 211

251 ± 502

  1. 1n ≥ 3; 2n ≥ 7; n.d. not determined.