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Table 1 FDH activities measured in the crude cell extracts of single- and co-expression strains at two induction temperatures.

From: Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

E coli strain Cb FDH (U/gCDW) at 25°C Cb FDH (U/gCDW) at 18°C
JM109_FDH 408 (reference, [8]) n.d.
BL21_FDH 402 ± 381 450
Star_FDH 458 551
Rosetta_FDH 445 614
BL21_XR_FDH 85 ± 122 110 ± 202
BL21_XR_2FDH 146 ± 301 186 ± 302
Rosetta_XR_FDH 100 ± 181 138 ± 232
Rosetta_XR_2FDH 202 ± 211 251 ± 502
  1. 1n ≥ 3; 2n ≥ 7; n.d. not determined.