Figure 3
From: Inclusion bodies as potential vehicles for recombinant protein delivery into epithelial cells
Epi-fluorescent microscopy of electroporated SW13 cells. (a) SW13 cells electroporated with a mixture of K14 ncIBs and a plasmid containing EYFP labeled K5 cDNA. (b) The positive control experiment, where cells were electroporated with plasmid DNAs encoding for EYFP-K5 and EYFP-K14. (C) The negative control experiment, where cells were electroporated exclusively with EYFP-K5 plasmid DNA. Experiments in (a) and (b) resulted in a multitude of speckles and short filamentous structures (see arrows). The scale bar is 20 μm.