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Figure 1 | Microbial Cell Factories

Figure 1

From: Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins

Figure 1

Overproduction of different secretory proteins in B. subtilis.(a) Whole cell extracts, (b) medium fractions, (c) membrane fractions, (d) cytoplasmic fractions. Left panels show SDS-PAGE gels; right panels (b, c and d) show immunodetection of the 6his-tagged proteins using Penta-His HRP conjugate antibodies (Qiagen). Asterisks indicate protein bands corresponding to the overproduced proteins. Calculated molecular masses of proteins, with and without signal peptide, in kDa: XynA, 32.3 (preprotein), 20.4 (matured); NprE, 56.5 (preprotein), 53.9 (matured); Bla (TEM-1 β-lactamase), 32.3 (preprotein), 29.7 (matured); Usp45, 48.2 (preprotein), 45.5 (matured); MntA, 33.4 (preprotein), 32.4 (matured); YcdH, 36.5 (preprotein), 34.3 (matured); LmrA, 66.2; XylP, 55.3.

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Microbial Cell Factories

ISSN: 1475-2859

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