Table 1 Bacterial strains and plasmids used in this study

Plasmids

pET-30a(+)

pBR322 ori lacI T7lac Kan^r

Novagen

pACYCDuet-1

P15A ori lacI T7lac Cm^r

Novagen

pCOLADuet-1

ColA ori lacI T7lac Kan^r

Novagen

pBAD/Myc-HisA

pBR322 ori ara BAD Amp^r

Invitrogen

pZh05

pACYCDuet-1 harboring FAR from Jojoba and fadD from E. coli

This study

pZh07

pACYCDuet-1 harboring acr1 from A. baylyi and fadD from E. coli

This study

pZh08

pACYCDuet-1 harboring CER4 from A. thaliana and fadD from E. coli

This study

pZh09

pACYCDuet-1 harboring FAR from Jojoba and yngl from B. subtilis

This study

pZh10

pACYCDuet-1 harboring FAR from Jojoba and FAA2 from S. cerevisiae

This study

pZh11

pACYCDuet-1 harboring acr1 from A. baylyi and yngl from B. subtilis

This study

pZh12

pACYCDuet-1 harboring acr1 from A. baylyi and FAA2 from S. cerevisiae

This study

pZh17

pCOLADuet-1 harboring acr1 from A. baylyi and fadD from E. coli

This study

pZh18

pCOLADuet-1 harboring acr1 from A. baylyi, fadD from E. coli and BTE from U. californica

This study

pZh27

pBAD/Myc-HisA harboring acr1 from A. baylyi and fadD from E. coli

This study

pYN2

pET-30a(+) harboring BTE from U. californica

26

pLL4

pCOLADuet-1 harboring ‘tesA from E. coli

This study

pYN12

pCOLADuet-1 harboring BTE from U. californica

This study

pXW2

pACYCDuet-1 harboring BTE from U. californica

This study

pYN22

pBAD/Myc-HisA harboring BTE from U. californica

This study

Strains

DH5α

F^-,φ80dlacZ ΔM15, Δ(lacZYA-argF)U169, deoR,recA1, endA1, hsdR17(rk^-,mk⁺), phoA, supE44, λ-,thi^-1, gyrA96, relA1

Takara

BL21 (DE3)

F^-ompT gal dcm lon hsdSB(r _B ^-m _B ^-) λ(DE3)

Invitrogen

Zh05

E. coli BL21 (DE3) bearing pZh05

This study

Zh07

E. coli BL21 (DE3) bearing pZh07

This study

Zh08

E. coli BL21 (DE3) bearing pZh08

This study

Zh072

E. coli BL21 (DE3) bearing pZh07 and pYN2

This study

Zh112

E. coli BL21 (DE3) bearing pZh11 and pYN2

This study

Zh122

E. coli BL21 (DE3) bearing pZh12 and pYN2

This study

Zh054

E. coli BL21 (DE3) bearing pZh05 and pLL4

This study

Zh094

E. coli BL21 (DE3) bearing pZh09 and pLL4

This study

Zh104

E. coli BL21 (DE3) bearing pZh10 and pLL4

This study

Zh0712

E. coli BL21 (DE3) bearing pZh07 and pYN12

This study

Zh172

E. coli BL21 (DE3) bearing pZh17 and pXW2

This study

Zh18

E. coli BL21 (DE3) bearing pZh18

This study

Zh0722

E. coli BL21 (DE3) bearing pZh07 and pYN22

This study

Zh1722

E. coli BL21 (DE3) bearing pZh17 and pYN22

This study

Zh272

E. coli BL21 (DE3) bearing pZh27 and pXW2

This study

Zh2712

E. coli BL21 (DE3) bearing pZh27 and pYN12

This study