Figure 5

Optimization of the expression level of C12/14 alcohol biosynthesis pathway. Plasmids with different copy numbers and promoters are used to optimize the expression level of C12/14 alcohol biosynthesis pathway. Zh172, with BTE and fadD-acr1 being carried by a higher copy number plasmid (T7 promoter) and a lower copy number plasmid (T7 promoter), respectively, achieved the highest C12/14 alcohol titer. Zh072 using two higher copy number plasmids (T7 promoter) accumulated a medium amount of C12/14 alcohol. Other strains produced a low concentration of C12/14 alcohol. The error bars represent the range from two independent experiments.