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Figure 6 | Microbial Cell Factories

Figure 6

From: Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

Figure 6

Enzymatic hydrolysis of barley straw and microcrystalline cellulose by BGLI- overexpressing transformants (T1-T4) and the parental strain ( T. reesei QM9414). (A and B) Reducing sugars and glucose yield (mg/mL) released using untreated barley straw, respectively. (C and D) Reducing sugars and glucose yield (mg/mL) released using NaOH- pretreated barley straw, respectively. (E and F) Reducing sugars and glucose yield (mg/mL) released using Organosolv-pretreated barley straw, respectively. (G and H) Reducing sugars and glucose yield (mg/mL) released using microcrystalline cellulose, respectively. 750 μl of the culture supernatants (as the enzyme source) were added to tubes containing 750 μl of 50 mM sodium citrate buffer (pH 5.0) and 0.045 g (3%) either barley straw or microcrystalline cellulose. The tubes were incubated at 50°C for 72 h and reducing sugars as well as glucose released were measured every 24 h. Data are represented as the mean of three independent experiments and error bars denote standard error of the mean. Total protein loading was as follow; T1: 0.22 mg; T2: 0.26 mg; T3: 0.24 mg; T4: 0.19 mg, and QM9414: 0.22 mg.

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