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Figure 4 | Microbial Cell Factories

Figure 4

From: Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

Figure 4

Identification of β-glucosidase in BGLI-overexpressing transformants (T1-T4) by MUG-zymogram assay. Proteins (culture supernatant) from BGLI-overexpressing transformants (T1-T4) and the parental strain (T. reesei QM9414, lane QM) were separated in 8% native PAGE. Culture supernatant of Periconia sp. (bgl1 donor strain) (lane P) grown on MA-medium containing 1% microcrystalline was used as the positive control. β-glucosidase activity was detected by MUG-zymogram assay. Lower arrow indicates the native extracellular β-glucosidase found in T. reesei strain (lanes T1-T4 and QM) whereas the upper arrow represents active β-glucosidase correlated to Periconia sp. BGLI only found in the BGLI- overexpressing transformants as well as Periconia sp. (lanes T1-T4 and P). Lane M, molecular weight marker.

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Microbial Cell Factories

ISSN: 1475-2859

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