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Figure 3 | Microbial Cell Factories

Figure 3

From: Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

Figure 3

The enzyme activities and time course study of BGLI-overexpressing transformants (T1-T4) and the parental strain ( T. reesei QM9414). The strains were pre- grown on MA-medium containing 1% glucose for 24 h and then mycelia were washed and grown for 144 h on MA-medium containing 1% microcrystalline for the induction. Enzyme activities were expressed as specific activities using international units per mg protein in the supernatant. (A) The β-glucosidase activity was measured every 24 h using the culture supernatant as the enzyme sources. The enzyme was incubated at 70°C for 10 min, pH 5.0 using 50 mM sodium citrate buffer. (B) Filter paper activity (FPA) was measured every 24 h using the culture supernatant and incubated at 50°C for 60 min, pH 4.8 using 75 mM citrate buffer. (C) Exoglucanases (Exo), and endoglucanases activities (EG) of the strains were measured after being induced with 1% microcrystalline cellulose for 120 h. No significant difference was obtained for Exo and EG activities of the four transformants compared to the parent strain usingone-way ANOVA at a confidence level of 99% (α = 0.01). Data are represented as the mean of three independent experiments and error bars denote standard error of the mean.

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