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Figure 2 | Microbial Cell Factories

Figure 2

From: Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

Figure 2

Construction of BGLI-overexpression T. reesei strain. (A) The structure of pPtef1- bgl1-cbh1 expression vector using SimVector software. Ptef1 represents tef1 promoter; bgl1 represents β-glucosidase gene from Periconia sp.; Tcbh1 indicates the cbh1 terminator; Pgpd1 represents gpd1 promoter (glyceraldehyde-3-phosphate dehydrogenase gene); hph represents hygromycin B phosphotransferase gene; Tgpd1 indicates the gpd1 terminator. (B) PCR assay ofthe bgl1 gene in the selected overexpression transformants using full-bgl1 primers. All transformants (T1-T4) showed a 2601 bp band whereas the parent strain (QM) did not amplified any specific PCR products. Periconia sp. genomic DNA (gP) as well as cDNA (cP) were used as the positive controls which amplified 2847 and 2601 bp products, respectively. (C) qPCR analysis of the isolated genomic DNA from the transformants T1-T4 using Real-Time bgl1 and tef1a primers. The transformants T1-T3 received one copy of the bgl1 gene whereas T4 obtained two copies.

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Microbial Cell Factories

ISSN: 1475-2859

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