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Table 1 The plasmids used in this research

From: Identification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent protein

Plasmids Characteristics Reference
pKK223-3 Cloning vector, Apr Amersham
pACYC-184 Cloning vector, Cmr New England Biolabs
pDSK-519 Cloning vector, Kmr [39]
pGFPuv gfp Clontech
pTOTAL P. fluorescens tliA operon [10]
pGFP-TliA gfp-tliA in pKK223-3 This study
pGFP-LARD1 to 5 gfp-lard1 to 5 in pKK223-3 This study
pEGF-LARD1 to 5 egf-lard1 to 5 in pKK223-3  
pEcPrtDEF prtDEF in pACYC-184 This study
pDSK-TliA tliA in pDSK-519 This study
pDSK-TliDEF (pDX) tliDEF in pDSK-519 This study
pDX-GFP-223 gfp and tliDEF in pDSK-519 This study
pDX-TliA tliA and tliDEF in pDSK-519 This study
pDX-GFP-TliA gfp-tliA and tliDEF in pDSK-519 This study
pDX-GFP-LARD3 gfp-lard3 and tliDEF in pDSK-519 This study