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Table 1 The plasmids used in this research

From: Identification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent protein

Plasmids

Characteristics

Reference

pKK223-3

Cloning vector, Apr

Amersham

pACYC-184

Cloning vector, Cmr

New England Biolabs

pDSK-519

Cloning vector, Kmr

[39]

pGFPuv

gfp

Clontech

pTOTAL

P. fluorescens tliA operon

[10]

pGFP-TliA

gfp-tliA in pKK223-3

This study

pGFP-LARD1 to 5

gfp-lard1 to 5 in pKK223-3

This study

pEGF-LARD1 to 5

egf-lard1 to 5 in pKK223-3

 

pEcPrtDEF

prtDEF in pACYC-184

This study

pDSK-TliA

tliA in pDSK-519

This study

pDSK-TliDEF (pDX)

tliDEF in pDSK-519

This study

pDX-GFP-223

gfp and tliDEF in pDSK-519

This study

pDX-TliA

tliA and tliDEF in pDSK-519

This study

pDX-GFP-TliA

gfp-tliA and tliDEF in pDSK-519

This study

pDX-GFP-LARD3

gfp-lard3 and tliDEF in pDSK-519

This study