The expression and secretion of the recombinant proteins in P. fluorescens ΔtliA . The intracellular expression and secretion of GFP-TliA and GFP-LARD3 were analyzed by western blotting using anti-LARD antibodies. The cells were isolated from the growth medium by double centrifugation. Lane 1, pDSK-TliA; lane 2, pDX-TliA; lane 3, pDX-GFP-LARD3; lane 4, pDX-GFP-TliA. The arrows indicate the sizes of GFP-LARD3 (38.2 kDa), TliA (49.9 kDa), and GFP-TliA (77.4 kDa).