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Figure 9 | Microbial Cell Factories

Figure 9

From: Identification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent protein

Figure 9

The expression and secretion of the recombinant proteins in P. fluorescens ΔtliA . The intracellular expression and secretion of GFP-TliA and GFP-LARD3 were analyzed by western blotting using anti-LARD antibodies. The cells were isolated from the growth medium by double centrifugation. Lane 1, pDSK-TliA; lane 2, pDX-TliA; lane 3, pDX-GFP-LARD3; lane 4, pDX-GFP-TliA. The arrows indicate the sizes of GFP-LARD3 (38.2 kDa), TliA (49.9 kDa), and GFP-TliA (77.4 kDa).

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