Figure 8From: Identification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent proteinThe expression and secretion of the recombinant proteins in P. fluorescens . A. The intracellular expression and secretion of GFP-TliA and GFP-LARD3 in P. fluorescens cultured in LB were detected by western blotting using anti-LARD antibodies. The P. fluorescens cells expressing the designated proteins with (+) or without (−) TliDEF were isolated from two different extracellular media by double centrifugation. B. The same expression was performed in 2× LB (double concentrated LB). Lane 1, pDSK-TliA; lane 2, pDX-TliA; lane 3, pDX-GFP-LARD3; lane 4, pDX-GFP-TliA. The arrows indicate the sizes of GFP-LARD3 (38.2 kDa), TliA (49.9 kDa), and GFP-TliA (77.4 kDa). Notice the intrinsic TliA indicated by the arrows.Back to article page