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Figure 8 | Microbial Cell Factories

Figure 8

From: Identification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent protein

Figure 8

The expression and secretion of the recombinant proteins in P. fluorescens . A. The intracellular expression and secretion of GFP-TliA and GFP-LARD3 in P. fluorescens cultured in LB were detected by western blotting using anti-LARD antibodies. The P. fluorescens cells expressing the designated proteins with (+) or without (−) TliDEF were isolated from two different extracellular media by double centrifugation. B. The same expression was performed in 2× LB (double concentrated LB). Lane 1, pDSK-TliA; lane 2, pDX-TliA; lane 3, pDX-GFP-LARD3; lane 4, pDX-GFP-TliA. The arrows indicate the sizes of GFP-LARD3 (38.2 kDa), TliA (49.9 kDa), and GFP-TliA (77.4 kDa). Notice the intrinsic TliA indicated by the arrows.

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