The extracellular secretion of GFP-LARDs in E. coli XL1-Blue and MM294. A. The secretion of GFP-LARDs in E. coli XL1-Blue. Each E. coli XL1-Blue culture harboring one of pGFP-LARD1-5 (lanes 1–5), pGFP-TliA (lane A) or pGFP-223 (lane None) with pEcPrtDEF (+) or pACYC-184 (−) was centrifuged twice, and the supernatant was collected. The growth conditions were identical to those listed in Figure 4. SDS-PAGE with 10% gel and western blotting with anti-LARD antibodies were performed on the supernatant. Purified TliA at a concentration of 99 μg/ml was used as an index after dilution. B. The secretion of GFP-LARDs in E. coli MM294. The methods used here were identical to those listed in A. The signs are also identical to those of A.