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Figure 4 | Microbial Cell Factories

Figure 4

From: Identification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent protein

Figure 4

The intracellular expression of GFP-LARDs in E. coli XL1-Blue. Each E. coli XL1-Blue culture harboring one of pGFP-LARD1-4 (lanes 1–4), pGFP-TliA (lane A) or pGFP-223 (lane None) with pEcPrtDEF (+) or pACYC-184 (−) was grown with 50 μg/ml ampicillin and 34 μg/ml chloramphenicol in a 2 ml glass tube at 37°C (IPTG was not added). The culture was centrifuged twice, and the pellet was isolated. SDS-PAGE with 10% gel and western blotting with anti-LARD antibodies were performed on the pellet. Purified TliA at a concentration of 99 μg/ml was used as an index after dilution.

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Microbial Cell Factories

ISSN: 1475-2859

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