The intracellular expression of GFP-LARDs in E. coli XL1-Blue. Each E. coli XL1-Blue culture harboring one of pGFP-LARD1-4 (lanes 1–4), pGFP-TliA (lane A) or pGFP-223 (lane None) with pEcPrtDEF (+) or pACYC-184 (−) was grown with 50 μg/ml ampicillin and 34 μg/ml chloramphenicol in a 2 ml glass tube at 37°C (IPTG was not added). The culture was centrifuged twice, and the pellet was isolated. SDS-PAGE with 10% gel and western blotting with anti-LARD antibodies were performed on the pellet. Purified TliA at a concentration of 99 μg/ml was used as an index after dilution.