Figure 7From: A novel expression system for production of soluble prion proteins in E. coliStructural superposition of the refolded and soluble MoPrP(89-230) 3D structures. Superimposition was generated with CS23D2.0 based on the backbone chemical shifts in green and orange respectively shown in two different orientations (A and B). The three helices α1, α2 and α3 are annotated for each structure. C) The structure of the refolded MoPrP(121-230) coloured in green exhibits a β-sheet between 128-130 and 161-163. Although this β-sheet is not present in the model of MoPrP(89-230) generated with CS23D2.0, the region Y156-Q167 that includes the second β-strand 161-163 in the refolded MoPrP(121-230) is well-superimposed with that in MoPrP(89-230) as highlighted by the blue circle.Back to article page