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Figure 1 | Microbial Cell Factories

Figure 1

From: A novel expression system for production of soluble prion proteins in E. coli

Figure 1

Effect of the co-expression of QSOX on the expression of soluble PrP in the cytoplasm of E. coli. A) SDS-PAGE analysis of MoPrP(89-230). Expression trials in presence of QSOX: Lane 1 = total E. coli lysate, Lane 2 = insoluble fraction, Lane 3 = soluble fraction. Expression trials in absence of QSOX: Lane 4 = total E. coli lysate, Lane 5 = insoluble fraction, Lane 6 = soluble fraction. The black arrow indicates the MoPrP(89-230). The corresponding immunoblot using anti-His-tag antibody is shown underneath the SDS-PAGE gel. B) SDS-PAGE and immunoblot analysis of HuPrP(90-231). C) SDS-PAGE and immunoblot analysis of MoPrP(23-230). D) SDS-PAGE and immunoblot analysis of HuPrP(23-231).

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