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Table 1 List of different test runs, the according gaseous composition and the method used to maintain pO 2 *

From: Methods to optimize myxobacterial fermentations using off-gas analysis

Low O2

Moderate CO2

Low O2

No CO2

Moderate O2

Moderate CO2

High O2

High CO2

High O2

No CO2

Moderate O2

High CO2

pO2 5%,

pO2 5%,

pO2 20%,

pO2 55%,

pO2 55%,

pO2 20%,

CO2 0.3–0.6%

CO2 < 0.1%

CO2 0.2–0.3%

CO2 1.0–2.0%

CO2 < 0.1%

CO2 1.8%

Shearing stress comparable to standard conditions

Shearing stress comparable to standard conditions

Standard conditions

Shearing stress comparable to standard conditions

Shearing stress comparable to standard conditions

New Method: Shearing stress dependent on stirrer speed

Gaseous composition and pO2 regulated by aeration with ambient air

Gaseous composition and pO2 regulated by aeration with ambient air stripping of CO2 with nitrogen gas

Gaseous composition and pO2 regulated by aeration with ambient air

Gaseous composition and pO2 regulated by aeration with pure O2

Gaseous composition and pO2 regulated by aeration with ambient air

Gaseous composition and pO2 regulated by aeration with ambient air

  1. *Trials were carried out in the presented order. Before and after the tests one standard run was carried out to ensure that no significant deviations are caused by the test runs.