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Table 1 List of different test runs, the according gaseous composition and the method used to maintain pO 2 *

From: Methods to optimize myxobacterial fermentations using off-gas analysis

Low O2
Moderate CO2
Low O2
No CO2
Moderate O2
Moderate CO2
High O2
High CO2
High O2
No CO2
Moderate O2
High CO2
pO2 5%, pO2 5%, pO2 20%, pO2 55%, pO2 55%, pO2 20%,
CO2 0.3–0.6% CO2 < 0.1% CO2 0.2–0.3% CO2 1.0–2.0% CO2 < 0.1% CO2 1.8%
Shearing stress comparable to standard conditions Shearing stress comparable to standard conditions Standard conditions Shearing stress comparable to standard conditions Shearing stress comparable to standard conditions New Method: Shearing stress dependent on stirrer speed
Gaseous composition and pO2 regulated by aeration with ambient air Gaseous composition and pO2 regulated by aeration with ambient air stripping of CO2 with nitrogen gas Gaseous composition and pO2 regulated by aeration with ambient air Gaseous composition and pO2 regulated by aeration with pure O2 Gaseous composition and pO2 regulated by aeration with ambient air Gaseous composition and pO2 regulated by aeration with ambient air
  1. *Trials were carried out in the presented order. Before and after the tests one standard run was carried out to ensure that no significant deviations are caused by the test runs.