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Table 5 Metabolic flux ratio (METAFoR) analysis results

From: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

 % Fraction of total pool 

X-33 control

 ROL  1- copy

 ROL  2-copy

X-33 control*

 

Glucose:Methanol

Glucose:Methanol

Glucose:Methanol

Glucose

Pep from methanol

45 ± 5

34 ± 9

24 ± 9

n.a.

Pep from PPP (upper bound)

14 ± 4

21 ± 8

24 ± 11

39 ± 9

R5P from T3P and S7P (transketolase)

77 ± 7

70 ± 1

83 ± 2

66 ± 2

R5P from E4P (transaldolase)

56 ± 1

57 ± 9

56 ± 4

40 ± 2

Ser originating from Gly and C1-unit

53 ± 3

55 ± 2

58 ± 1

62 ± 4

Gly originating from CO2 and C1-unit

7 ± 1

12 ± 1

8 ± 2

6 ± 4

Pep originating from Oaacyt (PepCK)

n.a.

n.a.

n.a.

2 ± 5

Oaa mit originating from Pep

49 ± 3

42 ± 12

36 ± 2

n.a.

  1. *Data taken from [26].
  2. METAFoR analysis results showing the origins of metabolic intermediates during growth of P. pastoris on glucose:methanol compared with those growing on glucose as the sole carbon source, n.a. (not available). Flux ratios used for MFA are highlighted in bold.