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Table 3 Biomass macromolecular composition for  P. pastoris 

From: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

B

Strain

Protein w/w

Carbohydrate w/w

Lipids* w/w

RNA* w/w

DNA* w/w

X-33 control

0.49 ± 0.02

0.31 ± 0.01

0.02 ± 0.02

0.07 ± 0.007

0.001 ± 0.0001

ROL 1-copy

0.43 ± 0.02

0.34 ± 0.01

0.04 ± 0.02

0.06 ± 0.007

0.001 ± 0.0001

ROL 2-copy

0.43 ± 0.03

0.34 ± 0.01

0.05 ± 0.02

0.07 ± 0.007

0.001 ± 0.0001

  1. * calculated values.
  2. Biomass macromolecular formula for P. pastoris growing on glucose:methanol mixture (80:20) as a carbon source in chemostat cultures at a D = 0.09 h−1. Data given as percentage of dry cell weight.
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Microbial Cell Factories

ISSN: 1475-2859

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