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Table 1 Growth parameters of  P. pastoris  growing on glucose:methanol in chemostats cultures

From: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

Strain

Glucose mmol/g CDW·h

Methanol mmol/g CDW·h

OUR mmol/g CDW·h

CER mmol/g CDW·h

Biomass mmol/g CDW·h

YX/S g CDW/Cmol

RQ

Lipase activity AU/gCDW

X-33 control

−0.76 ± 0.02

−1.15 ± 0.06

−2.95 ± 0.11

2.31 ± 0.11

3.40 ± 0.04

14.7 ± 0.3

0.78 ± 0.05

n.d.

ROL 1-copy

−0.85 ± 0.01

−1.29 ± 0.05

−3.71 ± 0.07

3.04 ± 0.06

3.32 ± 0.03

13.0 ± 0.3

0.82 ± 0.09

2504 ± 192

ROL 2-copy

−0.87 ± 0.01

−1.24 ± 0.09

−3.66 ± 0.06

3.04 ± 0.04

3.39 ± 0.05

12.9 ± 0.1

0.83 ± 0.07

3490 ± 208

  1. Reconciled measured substrates and products consumption or production rates at steady state conditions in each experiment. Substrate consumption, biomass and metabolites production rates for each strain. OUR, Oxygen Uptake Rate; CER, CO2 Exchange Rate; RQ Respiratory Quotient; n.d., not detectable. Consistency index h was below 7.81 for a redundancy of 3 (95% significance level) in all cases.