Figure 2From: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixturesMetabolic flux distributions in the P. pastoris reference and recombinant strains during growth on glucose:methanol. Metabolic flux distributions in the P. pastoris reference strain (top), the recombinant strain with 1 copy of the ROL gene (middle) and the recombinant strain harbouring 2 copies of the ROL gene (bottom) during growth on glucose:methanol chemostat cultures at about 0.09 h−1. Fluxes are normalized with respect glucose uptake flux (% C-mol/C-mol glucose). Activities of the malic enzyme and glyoxylate pathways were found to be negligible on the basis of the METAFoR analyses.Back to article page