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Table 3 Effects of co-expression of helper proteins on substrate protein activity

From: SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

 

Helper

vtPA

Urokinase

Chitinase

 

pBAD33

1.00

1.00

1.00

 

cAaDsbC

5.92

1.17

0.32

 

cDsbC

0.19

1.19

0.97

 

TrxACGPC

4.50

1.16

1.19

 

TrxACPYC

9.83

1.05

0.85

 

TrxACPHC

7.73

1.02

0.71

Redox active Helpers

cAaPDO

3.31

0.58

0.76

 

QSOX

8.12

0.86

1.02

 

PDI

3.08

1.52

(1.58)

 

EUG1

5.43

1.31

1.24

 

MPD1

4.88

1.10

1.42

 

MPD2

8.91

1.37

1.18

Chaperone helpers

MalE

0.87

1.11

1.13

 

HlpA (skp)

6.97

1.16

0.53

 

KatG

(11.81)

0.90

1.05

Oxidative stress helpers

AhpC, AhpF

5.69

(1.84)

0.90

 

AhpC*, AhpF

4.29

1.30

1.08

  1. Brackets indicates the best fold improvements while bold are the following best fold improvements.