SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Table 1 Table summarizing optimum expression conditions for all proteins produced in SHuffle

Substrate	#cysteine	Promoter	Optimum conditions			Yield mg/l	Specific Activity U/mg
			T °C	[IPTG] mM	Time of induction
vtPA N-HIS	12	T7	16	1.00	Mid log	0.2	721
			16	Auto	Autoexpression	1.2	159
Gluc	10	T7	37	1.00	Mid log	13.8	8.1E + 10
AppA	8	T7	37	0.01	Late log	51.3	6.74
Cel9A	6	T7	25	Auto	Autoexpression	250	320.4
PhoA	4	T7	25	0.05	Mid log	40.2	5.6E + 06
Chitinase	3	T7	16	0.10	Late log	7.1	4.5E + 09
CelZ	2	T7	25	Auto	Autoexpression	400	164.4

* Solid lines represent known disulfide bonds from crystal structures, dotted lines represent cysteines predicted to be involved in disulfide bonds with unknown structures.

ISSN: 1475-2859