Figure 6From: SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm Solubility of proteins expressed with optimal conditions in SHuffle. Cells were lysed and total cells lysates (T) were separated into soluble (S) and pellet (P) fractions. Samples were analyzed using western blot with the appropriate antibody and percentages of solubility are indicated. Anti-GroEL antibody was used as loading control.Back to article page