Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Figure 4

Redox state of DsbC. Redox state of AMS alkylated DsbC analyzed by western blot using anti-DsbC antibody. Redox states of DsbC are indicated as either hemi-reduced (hemi-red), reduced (red) or oxidized (oxi). (A) Redox state of periplasmic DsbC in wild type E. coli K12 (MB10; lane 1) and in E. coli B (C2566; lane 2), (B) Redox state of DsbC in the oxidizing E. coli ∆trxB, ∆gor, ahpC*, in the periplasm of E. coli K12 (MB932; lane 1), in E. coli B (MB1731; lane 2) and when expressed both in the periplasm and cytoplasm of SHuffle K12 (C3026; lane 3) or SHuffle B (C3029; lane 3).

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us