Figure 4From: SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm Redox state of DsbC. Redox state of AMS alkylated DsbC analyzed by western blot using anti-DsbC antibody. Redox states of DsbC are indicated as either hemi-reduced (hemi-red), reduced (red) or oxidized (oxi). (A) Redox state of periplasmic DsbC in wild type E. coli K12 (MB10; lane 1) and in E. coli B (C2566; lane 2), (B) Redox state of DsbC in the oxidizing E. coli ∆trxB, ∆gor, ahpC*, in the periplasm of E. coli K12 (MB932; lane 1), in E. coli B (MB1731; lane 2) and when expressed both in the periplasm and cytoplasm of SHuffle K12 (C3026; lane 3) or SHuffle B (C3029; lane 3).Back to article page