Figure 2
From: Using bacterial inclusion bodies to screen for amyloid aggregation inhibitors
Fluorescence recovery in the presence of metallic ions. (A) Aβ42wt-GFP IBs were denatured in 8 M Gu·HCl for 4 h and diluted 100-fold in PBS (control) or in PBS containing different metallic ions at 25 μM final concentration. (B) Purified untagged GFP and IBs were incubated in PBS in the absence (native) and presence of 8 M Gu·HCl for 4 h and diluted 100-fold in PBS containing Cu+2 and Zn+2 at 25 μM final concentration. In all cases, after incubation for 16 h fluorescence was recorded at 510 nm.