Figure 1
From: Using bacterial inclusion bodies to screen for amyloid aggregation inhibitors
Fluorescence recovery after denaturation. (A) Purified IBs were incubated in PBS in the absence (native) and presence of 8 M Gu·HCl or 10 M urea for 4 h and diluted 100-fold in PBS. (B) Purified untagged GFP (left) and IBs (right) were incubated in PBS in the absence (native) and presence of 8 M Gu·HCl or 10 M urea for 4 h and diluted 100-fold in PBS. In all cases, after incubation for 16 h fluorescence was recorded at 510 nm.