Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man8GlcNAc2 and Man5GlcNAc2

Table 1 PCR primers and oligonucleotides

Primer name	Sequence (5’… 3’)	Restriction site
OCH1Pfw	TCGCTATCACGTCTCTAGC
OCH1Prv	CTAGGGATAACAGGGTAAT GGTGTGACGAAGTATCGAG	I-Sce I
OCH1Tfw	CATTACCCTGTTATCCCTA GCGAGATCATGGACTGG	I-Sce I
OCH1Trv	ACTCTGTATACTTGTATGTATGTACTG
MNN9Pfw	TGAGCGGCCG CTTTTCTACTTCAGAGCTGGAG	Not I
MNN9Prv	TAGGGATAACAGGGTAAT CTTGAATGAGTATGTGTCGTG	I-Sce I
MNN9Tfw	ATTACCCTGTTATCCCTA GAAGGAGATGTAGCGTAAG	I-Sce I
MNN9Trv	GGCTTAATTAA TTGGTAGTGATATAATGTAACGC	Pac I
LIP2prefw	GATCCATGAAGCTTTCCACCATCCTCTTCACAGCCTGCGCTACCCTGGCCGCGGTAC
LIP2prerv	CTAGGTACCGCGGCCAGGGTAGCGCAGGCTGTGAAGAGGATGGTGGAAAGCTTCATG
ManHDELfw	GGCAGCGCT ACAAAACGTGGATCTCCCAAC	Eco47 III
ManHDELrv	GGCCCTAGG TTACAACTCGTCGTGAGCAAG	Avr II

ISSN: 1475-2859