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Figure 5 | Microbial Cell Factories

Figure 5

From: Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man8GlcNAc2 and Man5GlcNAc2

Figure 5

DSA-FACE analysis of N-glycans derived from different Yarrowia lipolytica strains. (A) Oligomaltose reference. (B-L) Different N-glycans. (B) Bovine RNaseB reference. (C) The MTLY60 wild type strain. (D-E) HDEL-tagged α-1,2-mannosidases expressed in a Δoch1 background: (D) the mannosidase fused to the S. cerevisiae α-mating factor prepro sequence and (E) the mannosidase fused to the Y. lipolytica LIP2pre. (F-L) The codon-optimized mannosidase fused to the Y. lipolytica LIP2pre: (F) under control of the hp4d promoter and grown in YPD, (G) under control of the GAP promoter and grown in YPD, (H) under control of the TEF promoter and grown in YPD, (I) under control of the hp4d promoter and grown in YTO, (J) under control of the GAP promoter and grown in YTO, (K) under control of the TEF promoter and grown in YTO, (L) under control of the POX promoter and grown in YTO.

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Microbial Cell Factories

ISSN: 1475-2859

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