Figure 4

Western blot evaluation of hypermannosylation of glucocerebrosidase before and after inactivation of YlOCH1 . Expression of glucocerebrosidase by the wild-type strain (WT, MTLY60) (lane1) and by the Δoch1 mutant strain (lane2). The WT strain shows a smear, indicating hyperglycosylation, while the Δoch1 mutant strain shows a distinct band above 55 kDa, which is the MW of glucocerebrosidase without sugars.