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Figure 2 | Microbial Cell Factories

Figure 2

From: De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia Lipolytica

Figure 2

Relative copy numbers of integrated expression cassettes in Yarrowia lipolytica multi-copy transformants. Real-time PCR was used to estimate the copy number of the integrated expression cassettes among 13 Polh-1292-oPAI transformants. Y. lipolytica Polg was used as a control organism with a single copy of both the URA3 and SUC2 target sequences. As URA3 and oPAI coexisted in expression cassette, the copy numbers of both genes were considered as equal.

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Microbial Cell Factories

ISSN: 1475-2859

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