A. Physical map of the expression cassettes that are present in strains T7-36 (plasmid pHGChyC), TA-9 (pAQF), TAPL-4 (plasmid pAPLQF), TG-87 (pGlaQF) and TGPL-10 (pGLaPLQF). The synthetic chymosin gene is flanked by the promoter region of the gpdA (in the original strain T7-36), amyB or glaA genes, which are indicated by a black arrow. Protein secretion signals are indicated as a shaded box (pre sequence of the pre-prochymosin) or an open box (PL, leader peptide of α-amylase or glucoamylase). The Tcyc1 transcriptional terminator from S. cerevisiae is represented as a black box. B. Time course of chymosin production, growth and pH profiles of the above-mentioned strains cultured in flasks containing MDF3 medium (including 10 g/L casein). Error bars indicate the standard deviation of three analyses made from three independent cultures.