Figure 1From: Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori. Proteomics studies reveal changes in the secretory pathwayA. Physical map of the expression cassettes that are present in strains T7-36 (plasmid pHGChyC), TA-9 (pAQF), TAPL-4 (plasmid pAPLQF), TG-87 (pGlaQF) and TGPL-10 (pGLaPLQF). The synthetic chymosin gene is flanked by the promoter region of the gpdA (in the original strain T7-36), amyB or glaA genes, which are indicated by a black arrow. Protein secretion signals are indicated as a shaded box (pre sequence of the pre-prochymosin) or an open box (PL, leader peptide of α-amylase or glucoamylase). The Tcyc1 transcriptional terminator from S. cerevisiae is represented as a black box. B. Time course of chymosin production, growth and pH profiles of the above-mentioned strains cultured in flasks containing MDF3 medium (including 10 g/L casein). Error bars indicate the standard deviation of three analyses made from three independent cultures.Back to article page