Table 2 Strains and plasmids used in this study
Strain, plasmid, or primer | Relevant features | Source or reference |
|---|---|---|
Strains | ||
Escherichia coli strains | ||
Nova blue | endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB+lacIq ZΔM15::Tn10(Tetr)] | Novagen |
S17-1 λpir | TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir | BIOMEDAL |
Streptomyces maritimus | ||
/WT | DSMZ 41777 WT strain | DSMZ |
/ps-tfu0901 | DSMZ 41777 strain with tfu0901-secreting expression vector | This study |
/ps-tfu1074 | DSMZ 41777 strain with tfu1074-secreting expression vector | This study |
This study | ||
Streptomyces lividans 1326 | WT strain | NBRC |
Streptomyces coelicolor A3(2) | WT strain | NBRC |
Plasmids | ||
pUC702-prom-sig-term | Versatile vector for protein expression; thiostrepton resistance marker; pld promoter; rep, replication gene from pIJ101; size, 8,600 bp | 25 |
pUC702-ps-Tfu0901-(His)6 | Vector for secreting endoglucanase (Tfu0901); thiostrepton resistance marker; pld promoter; rep, replication gene from pIJ101; size, 9,900 bp | This study |
pUC702-ps-Tfu1074-(His)6 | Vector for secreting endoglucanase (Tfu1074); thiostrepton resistance marker; pld promoter; rep, replication gene from pIJ101; size, 9,800 bp | 25 |
pTONA4 | Versatile vector for protein expression; kanamycin and thiostrepton resistance marker; pld promoter; rep, replication gene from pIJ101; size, 9,000 bp | This study |
pTONA4-ps-tfu0901 | Vector for secreting endoglucanase (Tfu0901); kanamycin and thiostrepton resistance marker; pld promoter; rep, replication gene from pIJ101; size, 10,300 bp | This study |
pTONA4-ps-tfu1074 | Vector for secreting endoglucanase (Tfu1074); kanamycin and thiostrepton resistance marker; pld promoter; rep, replication gene from pIJ101; size, 10,200 bp | This study |