Table 2 Summary of batch fermentations of E . coli strains producing 2-FL from lactose
Strains | Plasmids | Initial lactose concentration (g/l) | Maximum dry cell mass (g/l) | Maximum 2-FL concentration (g/l) | Yield (g 2-FL/g lactose) |
|---|---|---|---|---|---|
BL21star(DE3) | pmBCGW + pHfucT2 | 2.56 ± 0.04 | 1.84 ± 0.05 | 0.01 ± 0.001 | 0.005 ± 0.001 |
JM109(DE3) | pmBCGW + pHfucT2 | 2.55 ± 0.02 | 1.17 ± 0.05 | 0.14 ± 0.015 | 0.06 ± 0.005 |
14.54 ± 0.67 | 1.70 ± 0.28 | 1.23 ± 0.011 | 0.09 ± 0.004 |