Table 1 List of primers and plasmids used in this study
Name | Sequence of PCR primers and description for plasmids | Source |
|---|---|---|
PCR primers | ||
fucT2_F (Nco I) | 5′-ACATGCCATGG CTTTTAAGGTGGTGCAA-3′ | H. pylori 26695 (ATCC 700392) |
fucT2_R (Sac I) | 5′-AGTCCGAGCTC TTAAGCGTTATACTTTTGGGA-3′ | |
Plasmids | ||
pETDuet-1 | two T7 promoters with two MCS, pBR322 replicon (copy number ~40), Ampr | Merck Biosciences |
pCOLADuet-1 | two T7 promoters with two MCS, ColA replicon (copy number 10 ~ 12), Kanr | Merck Biosciences |
pmBCGW | derived from pETDuet-1, PT7-manB-manC ( Nco I/ Sac I)-PT7-gmd-wcaG ( Nde I/ Xho I)-TT7, Ampr | Lee et al., 2009 |
pHfucT2 | derived from pCOLADuet-1, PT7- fucT2 ( Nco I/ Sac I)-PT7-MCS2-TT7, Kanr | this study |