Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli

Figure 4

LC/MS analysis of 2-FL biosynthesis in the batch fermentation of the recombinant E . coli JM109(DE3) overexpressing ManB, ManC, Gmd, WcaG and FucT2. At the end of batch fermentation, culture broth was collected for confirmation of extracellular 2-FL production. HPLC analysis of 100 mg/l 2-FL standard solution ( A), HPLC analysis of culture broth of E. coli JM109(DE3) harboring pmBCGW + pHfucT2 ( B) and MS analysis of the compound with the retention time = 6.6 min in the culture broth of E. coli JM109(DE3) harboring pmBCGW + pHfucT2 ( C).

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us