Table 3 Primers used in PCR and DNA sequencing reactions. F: forward primer, R: reverse primer, underlined: restriction endonuclease site
Name | Sequence (5′-3′) | Application |
|---|---|---|
FwtdpgA | CG GGATCC ACCATGTCGATCCTTAACG | Amplifying and cloning |
RwtdpgA | AG GAATTC TCATGATTGGTTTCCAGAC | Â |
FdpgA _3.5 K | CG GGATCC ACCATGTCCATCCTGAACG |  |
RdpgA _3.5 K | AG GAATTC TTAGCAGTGATGGTGAT |  |
FdpgA _AO | G GAATTC ACCATGTCCATCCTGAACGACTACAAGAG | Â |
RdpgA _AO | CA GGTCTCCAATT GTTAGCAGTGATGGTGATGGTGATG | Â |
FgroES _GAP | G GAATTC ACCATGAATATTCGTCCATTGC | Â |
RgroES _GAP | GC TCTAGA TTACGCTTCAACAATTGCCAG | Â |
FgroEL _GAP | G GAATTC ACCATGGCAGCTAAAGACGTAAAATTCGGTAACG | Â |
RgroEL _GAP | GC TCTAGA TTACATCATGCCGCCCATGCCACC | Â |
FgroES _AO | G GAATTC ACCATGAATATTCGTCCATTGC | Â |
RgroES _AO | CA GGTCTCCAATT GTTACGCTTCAACAATTGCCAG | Â |
FgroEL _AO | G GAATTC ACCATGGCAGCTAAAGACGTAAAATTCGGTAACG | Â |
RgroEL _AO | CA GGTCTCCAATT GTTACATCATGCCGCCCATGCCACC | Â |
F_ELS_PICZ | CCG CTCGAG TTTTGGTCATGCATGAGATC | Â |
R_ELS_PICZ | ATAGTTTA GCGGCCGC TTTTGAAGCTATGGTGTG | Â |
F_GroEL | TGTCCGTACCATGCTCTGAC | Copy number determination |
R_GroEL | CAGGTAGCCACGGTCGAAC | Â |
F_D-PhgAT | GCCTGCTCCAGGTGTCTTGC | Â |
R_D-PhgAT | ACTGTCTAGCCAATTCAGCACC | Â |
F_Actin | AAAAGATCTGGCACCACACC | Â |
R_Actin | AGTGGTTCTACCGGAAGCG | Â |
5′AOX1 | GACTGGTTCCAATTGACAAGC | DNA sequencing |
3′AOX1 | GCAAATGGCATTCTGACATCC |  |