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Table 2 Effects of cultivation conditions on D-PhgAT yield from P. pastoris strain KM_AT3_ELS10

From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Cultivation condition D-PhgAT activity
Temperature Additive Specific activity [U·mg-1]a Volumetric activity [U·L-1]b Volumetric productivity[U·L-1·h-1]
  0.5 M Sorbitol 0.4 mM Benzyl alcohol    
30°C - - 60.1 41730 1738.75
  + - 52.3 36526 1521.92
  - + 52.7 36616 1525.67
  + + 51.1 35504 1479.33
25°C - - 72.7 50512 2104.67
  + - 70.1 48663 2027.63
  - + 72.0 50165 2090.21
  + + 67.1 46602 1941.75
20°C - - 58.9 40810 1700.42
  + - 53.1 36920 1538.33
  - + 58.3 40830 1701.25
  + + 51.7 35722 1488.42
  1. a,bSee Table 1.