Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Table 2 Effects of cultivation conditions on D-PhgAT yield from P. pastoris strain KM_AT3_ELS10

Cultivation condition			D-PhgAT activity
Temperature	Additive		Specific activity [U·mg^-1]^a	Volumetric activity [U·L^-1]^b	Volumetric productivity[U·L^-1·h^-1]
	0.5 M Sorbitol	0.4 mM Benzyl alcohol
30°C	-	-	60.1	41730	1738.75
	+	-	52.3	36526	1521.92
	-	+	52.7	36616	1525.67
	+	+	51.1	35504	1479.33
25°C	-	-	72.7	50512	2104.67
	+	-	70.1	48663	2027.63
	-	+	72.0	50165	2090.21
	+	+	67.1	46602	1941.75
20°C	-	-	58.9	40810	1700.42
	+	-	53.1	36920	1538.33
	-	+	58.3	40830	1701.25
	+	+	51.7	35722	1488.42

ISSN: 1475-2859