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Figure 9 | Microbial Cell Factories

Figure 9

From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Figure 9

Purification of the C-terminally 6xHis-tagged D-PhgAT by affinity chromotagraphy. (a) Protein elution profile of D-PhgAT purification from cell-free extract by immobilized ion affinity chromatography; (b) SDS-PAGE analysis of eight 3 mL active fractions collected during the elution volume of 130–154 mL showing purity and amount of D-PhgAT (45 kDa) obtained; (c) SDS-PAGE analysis of proteins in cell-free extract (Lane 1), unbound fraction (Lane 2), weakly bound fraction (Lane 3), and the purified concentrated D-PhgAT (Lane 4). Lane M, molecular mass markers.

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