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Figure 8 | Microbial Cell Factories

Figure 8

From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Figure 8

Comparison of D-PhgAT expressions from P. pastoris KM_AT3_ELS10 cultivated under different conditions for 24 h. SDS-PAGE analysis of soluble and insoluble protein fractions of the cultures induced at 30°C (a), 25°C (b) and 20°C (c), respectively, without and with 0.5 M sorbitol or 0.4 mM benzyl alcohol or both added. Lane M, molecular mass markers; the protein bands corresponding to D-PhgAT (45 kDa) are arrow indicated.

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