Figure 8From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroESComparison of D-PhgAT expressions from P. pastoris KM_AT3_ELS10 cultivated under different conditions for 24 h. SDS-PAGE analysis of soluble and insoluble protein fractions of the cultures induced at 30°C (a), 25°C (b) and 20°C (c), respectively, without and with 0.5 M sorbitol or 0.4 mM benzyl alcohol or both added. Lane M, molecular mass markers; the protein bands corresponding to D-PhgAT (45 kDa) are arrow indicated.Back to article page