Figure 5From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroESD-PhgAT activity from P. pastoris KM71 co-expressing groEL and groES with dpgA. D-PhgAT activity in cell extract and viable cell counts of P . pastoris KM71 integrated with pAO_D-PhgAT_AOX_ELS plasmid (white circle, white triangle) or pAO_D-PhgAT_GAP_ELS (filled circle, filled triangle) during induction with 0.5% methanol at 30°C for 72 h. Solid line, specific activity; dash line, volumetric activity; please see Table 1 for definitions.Back to article page