Figure 3From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroESP. pastoris KM71 expressed D-PhgAT as insoluble protein. SDS-PAGE analysis of soluble (S) and insoluble (I) protein fractions of P. pastoris KM71 expressing D-PhgAT under the control of AOX1 promoter after induction at 30°C for 24 h. Lane M, molecular mass markers. The arrow indicates protein band of the D-PhgAT (45 kDa).Back to article page