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Figure 2 | Microbial Cell Factories

Figure 2

From: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

Figure 2

Expression of D-PhgAT in P. pastoris KM71. SDS-PAGE analysis of total cellular proteins of (a) P. pastoris KM71 expressing wild-type and codon-optimized dpgA genes under the control of AOX1 promoter at 30°C for 24 h. Lane M, molecular mass markers; Lane 1, P. pastoris KM71 harboring the parent vector pPIC3.5 K; Lane 2, P. pastoris strain KM_AT3 (see Table 1) expressing D-PhgAT from the codon-optimized dpgA gene; Lane 3–5, three P. pastoris clones expressing D-PhgAT from the wild-type dpgA gene. (b) Lane 2–7, P. pastoris KM_AT3 after induction at 30°C for 24, 48, 72, 96, 120 and 144 h, respectively. The arrow indicates protein bands of the D-PhgAT (45 kDa).

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