New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol

Table 8 Oligonucleotides utilized in this report (gene inactivations and RT-qPCR)

A. Oligonucleotides used for the detection of gene inactivations
pckA	pckAFw	CAG GAA TGC GAT TCC ACT CA
pckA	pckARv	GTG CAG CGT ATC GTG GAT AA
ppc	ppcFw	GCA TCT TAT CCG ACC TAC AC
ppc	ppcRv	GCC TGT AGC AGA GTA GAG AA
pykA	pykAFw	CTG AAG GAA TCG CGT CGT TTT GA
pykA	pykARv	CGG CGG ATG AAT GAA GAA
pykF	pykFor	ACA AGC ACA CAT TCC TCT GCA
pykF	pykRev	AAA ACA GGA TGC TTC CAT CG
tnaA	tnaAFw	TTC TGT AGC CAT CAC CAG AG
tnaA	tnaARv	CCG GCA AGA TCA ACA GGT AA
galP	galpAa	CAT GTA TTA CGC GCC GAA AA
galP	galpAb	TGG CAA GTA CGT TGG TCA GG
B. Oligonucleotides used for RT-qPCR assays*
cpdA	cpdAa	CAG CAT TTC GCT GAA GGC AT
cpdA	cpdaB	GCA TCC TGT AAC GCG CTG TAC
glpD	glpDa	ATG GTG CTG GTA TCG CGG
glpD	glpDb	TTT TGA ACT GGC GGA AGA GG
glpF	glpFa	AGG CCA GTG CAT TGC TGA AT
glpF	glpFb	ACT GAC CAA AAG ACG CAC CAG
glpK	glpKa	CTC GAC CAT GTG GAA GGC TC
glpK	glpKb	ACA CGG CCC TGA GTC ATT TT
pntA	pntAa	AAC CAG CGC CGA AGC TAA TT
pntA	pntAb	GTA TTC ACA GTT GCC GCC GT
udhA	udhAa	AAG GCT GTG ACG ATG GTG TG
udhA	udhAb	CGA ATC GGT ATT ACC GGT GC

* Oligonucleotide sequences of the remaining utilized genes in Table 3 have been published elsewhere [12, 13, 19, 20, 68].

ISSN: 1475-2859