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Table 8 Oligonucleotides utilized in this report (gene inactivations and RT-qPCR)

From: New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol

A. Oligonucleotides used for the detection of gene inactivations
pckA pckAFw CAG GAA TGC GAT TCC ACT CA
pckARv GTG CAG CGT ATC GTG GAT AA
ppc ppcFw GCA TCT TAT CCG ACC TAC AC
ppcRv GCC TGT AGC AGA GTA GAG AA
pykA pykAFw CTG AAG GAA TCG CGT CGT TTT GA
pykARv CGG CGG ATG AAT GAA GAA
pykF pykFor ACA AGC ACA CAT TCC TCT GCA
pykRev AAA ACA GGA TGC TTC CAT CG
tnaA tnaAFw TTC TGT AGC CAT CAC CAG AG
tnaARv CCG GCA AGA TCA ACA GGT AA
galP galpAa CAT GTA TTA CGC GCC GAA AA
galpAb TGG CAA GTA CGT TGG TCA GG
B. Oligonucleotides used for RT-qPCR assays*
cpdA cpdAa CAG CAT TTC GCT GAA GGC AT
cpdaB GCA TCC TGT AAC GCG CTG TAC
glpD glpDa ATG GTG CTG GTA TCG CGG
glpDb TTT TGA ACT GGC GGA AGA GG
glpF glpFa AGG CCA GTG CAT TGC TGA AT
glpFb ACT GAC CAA AAG ACG CAC CAG
glpK glpKa CTC GAC CAT GTG GAA GGC TC
glpKb ACA CGG CCC TGA GTC ATT TT
pntA pntAa AAC CAG CGC CGA AGC TAA TT
pntAb GTA TTC ACA GTT GCC GCC GT
udhA udhAa AAG GCT GTG ACG ATG GTG TG
udhAb CGA ATC GGT ATT ACC GGT GC
  1. * Oligonucleotide sequences of the remaining utilized genes in Table 3 have been published elsewhere [12, 13, 19, 20, 68].