New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol

Table 4 Proteins differentially produced in strain JM101 grown on glycerol, as compared to the production on glucose

Spot No.	Gene name	Protein description	Mw	pI	Ratio IODglycerol/IODglucose
B11	argG	Argininosuccinate synthase	50038	5.23	0.37
58	cdd	Cytidine deaminase	31805	5.42	6.97
51	fumA	Fumarate hydratase class I	60774	6.11	2.38
57	mglB	D-galactose-binding periplasmic protein	35690	5.68	81.03
11	glpD	Aerobic glycerol-3-phosphate dehydrogenase	56886	6.97	5.18
D11	glpK	Glycerol kinase	56480	5.36	16.18
75	glnA	Glutamine synthetase	52099	5.26	0.31
60	argT	Lysine-arginine-ornithine-binding periplasmic protein	28088	5.62	2.57
P10	ompC	Outer membrane protein C	40343	4.58	0.32
31	malE	Maltose-binding periplasmic protein	43360	5.53	7.07
27	lamB	Maltoporin	49995	4.81	6.94
50	pckA	Phosphoenolpyruvate carboxykinase [ATP]	59891	5.46	9.17
73	ptsI	Phosphoenolpyruvate-protein phosphotransferase	63750	4.78	0.23
D10	deoD	Purine nucleoside phosphorylase deoD-type	26161	5.42	2.08
63	gatY	D-tagatose-1,6-bisphosphate aldolase subunit	31021	5.87	55.83
62	udp	Uridine phosphorylase	27313	5.81	7.41
K10	tnaA	Tryptophanase	53119	5.88	17.03
72	typA	GTP-binding protein TypA/BipA	67542	5.16	0.44

Spot intensities were quantified using PD Quest software 8.0.1 on three experiments. Only reproducible phenotypes, with a Student’s t test value p ≤ 0.05 are shown. Additional file 3 contains other important proteomic parameters.
pI: Isoelectric point.
Mw: Molecular weight.
IOD: Integrated optical density.

ISSN: 1475-2859