Skip to main content

Advertisement

Table 4 Proteins differentially produced in strain JM101 grown on glycerol, as compared to the production on glucose

From: New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol

Spot No. Gene name Protein description Mw pI Ratio IODglycerol/IODglucose
B11 argG Argininosuccinate synthase 50038 5.23 0.37
58 cdd Cytidine deaminase 31805 5.42 6.97
51 fumA Fumarate hydratase class I 60774 6.11 2.38
57 mglB D-galactose-binding periplasmic protein 35690 5.68 81.03
11 glpD Aerobic glycerol-3-phosphate dehydrogenase 56886 6.97 5.18
D11 glpK Glycerol kinase 56480 5.36 16.18
75 glnA Glutamine synthetase 52099 5.26 0.31
60 argT Lysine-arginine-ornithine-binding periplasmic protein 28088 5.62 2.57
P10 ompC Outer membrane protein C 40343 4.58 0.32
31 malE Maltose-binding periplasmic protein 43360 5.53 7.07
27 lamB Maltoporin 49995 4.81 6.94
50 pckA Phosphoenolpyruvate carboxykinase [ATP] 59891 5.46 9.17
73 ptsI Phosphoenolpyruvate-protein phosphotransferase 63750 4.78 0.23
D10 deoD Purine nucleoside phosphorylase deoD-type 26161 5.42 2.08
63 gatY D-tagatose-1,6-bisphosphate aldolase subunit 31021 5.87 55.83
62 udp Uridine phosphorylase 27313 5.81 7.41
K10 tnaA Tryptophanase 53119 5.88 17.03
72 typA GTP-binding protein TypA/BipA 67542 5.16 0.44
  1. Spot intensities were quantified using PD Quest software 8.0.1 on three experiments. Only reproducible phenotypes, with a Student’s t test value p ≤ 0.05 are shown. Additional file 3 contains other important proteomic parameters.
  2. pI: Isoelectric point.
  3. Mw: Molecular weight.
  4. IOD: Integrated optical density.