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Table 3 Relative transcription levels determined by RT-qPCR of several group of genes from strain JM101 on glycerol as the only carbon source

From: New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol

Glycolysis

Gluconeogenic, anaplerotic and glyoxylate shunt

TCA cycle

aceE 0.67 +/- 0.17

aceA 1.97 +/- 0.39

acnA 1.58+/- 0.26

aceF 0.80 +/- 0.22

aceB 1.95 +/- 0.06

acnB 4.71+/- 0.47

eno 0.86 +/- 0.12

ackA 1.49 +/- 0.28

fumA 2.44+/- 0.45

fbaA 1.52 +/- 0.09

acs 4.16 +/- 0.34

fumB 7.49+/- 0.40

fbaB 2.76 +/- 0.22

fbp 1.51 +/- 0.10

fumC 0.34+/- 0.10

gapA 0.72 +/- 0.06

glcB 1.81 +/- 0.56

gltA 0.76+/- 0.02

gapC-1 1.31 +/- 0.10

glcC 1.81 +/- 0.28

icd 0.47+/- 0.10

gapC-2 3.19 +/- 0.67

maeB 1.23 +/- 0.01

mdh 0.65+/- 0.10

glk 3.81 +/- 0.14

pckA 5.56 +/- 1.77

sdhA 1.04+/- 0.01

gpmA 0.90 +/- 0.09

poxB 7.33 +/- 0.56

sdhB 1.37+/- 0.42

lpdA 0.56 +/- 0.05

ppc 1.11 +/- 0.14

sdhC 0.92+/- 0.05

pfkA 1.91 +/- 0.22

pta 2.10 +/- 0.23

sdhD 1.13+/- 0.21

pfkB 2.15 +/- 0.01

ppsA 1.26 +/- 0.22

sucA 0.81+/- 0.13

pgi 1.58 +/- 0.37

sfcA 2.05 +/- 0.34

sucB 0.80+/- 0.23

pgk 1.08 +/- 0.13

 

sucC 0.79+/- 0.07

pykA 1.77 +/- 0.10

 

sucD 1.36+/- 0.13

pykF 0.98 +/- 0.09

  

tpi 1.36 +/- 0.10

  

Pentose phosphate Entner-Doudoroff

Glycerol uptake

mal/lam and mgl/gal regulons

eda 1.83+/-0.29

glpF 7.16 +/- 0.15

galP 5.05+/-0.26

edd 4.45+/-0.29

glpK 5.60 +/- 0.87

galR 0.38+/-0.13

gnd 0.80+/-0.00

glpD 1.26 +/- 0.25

galS 0.57+/-0.01

talA 1.64+/-0.47

glpR 2.40 +/- 0.06

galT 0.49+/-0.00

talB 0.60+/-0.01

 

lamB 1.36+/-0.30

tktA 1.22+/-0.06

 

mglB 5.05+/-0.58

tktB 0.70+/-0.11

 

mglC 2.28+/-0.12

zwf 1.34+/-0.04

 

malE 1.77+/-0.20

  

pgm 2.37+/-0.62

Aromatic pathway

Respiration, transhydrogenases

Transporters/ porins

aroA 0.81 +/- 0.03

frdA 1.58+/-0.15

actP 13.49+/-1.31

aroB 1.98 +/- 0.59

frdB 1.73+/-0.11

aroP 1.61+/-0.00

aroC 1.34 +/- 0.02

frdC 4.68+/-0.21

shiA 0.75+/-0.02

aroD 3.29 +/- 0.07

frdD 2.01+/-0.34

ompC 0.17 +/- 0.01

aroE 5.47 +/- 0.16

pntA 0.69+/-0.03

ompF 1.17 +/- 0.21

aroF 1.32 +/- 0.08

udhA 4.89+/-0.88

 

aroG 0.50 +/- 0.13

  

aroH 1.36 +/- 0.13

  

aroK 1.01 +/- 0.07

  

aroL 2.48 +/- 0.09

  

Regulators

  

arcA 1.25+/-0.29

  

arcB 4.45+/-0.56

  

creB 3.19+/-0.55

  

creC 6.27+/-1.69

  

crp 1.71+/-0.12

  

csrA 1.03+/-0.14

  

csrB 0.48+/-0.10

  

cyaA 1.64+/-0.24

  

fadR 1.22+/-0.31

  

fruR 3.00+/-0.20

  

iclR 0.29+/-0.10

  

ihfA 1.15+/-0.25

  

mlc 1.18+/-0.15

  

pdhR 0.57+/-0.19

  

ptsG 0.14 +/-0.02

  

rpoS 1.04+/-0.22

  
  1. Transcription levels of the measured genes of strain JM101 grown on glucose were used as controls to normalize (as one) the data using the RT-qPCR value for the corresponding gene. Results presented are the averages of at least three independent measurements of the RT-qPCR expression values for each gene. Expression levels are presented as 2−ΔΔCT values (see Methods).
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Microbial Cell Factories

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