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Table 3 Relative transcription levels determined by RT-qPCR of several group of genes from strain JM101 on glycerol as the only carbon source

From: New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol

Glycolysis Gluconeogenic, anaplerotic and glyoxylate shunt TCA cycle
aceE 0.67 +/- 0.17 aceA 1.97 +/- 0.39 acnA 1.58+/- 0.26
aceF 0.80 +/- 0.22 aceB 1.95 +/- 0.06 acnB 4.71+/- 0.47
eno 0.86 +/- 0.12 ackA 1.49 +/- 0.28 fumA 2.44+/- 0.45
fbaA 1.52 +/- 0.09 acs 4.16 +/- 0.34 fumB 7.49+/- 0.40
fbaB 2.76 +/- 0.22 fbp 1.51 +/- 0.10 fumC 0.34+/- 0.10
gapA 0.72 +/- 0.06 glcB 1.81 +/- 0.56 gltA 0.76+/- 0.02
gapC-1 1.31 +/- 0.10 glcC 1.81 +/- 0.28 icd 0.47+/- 0.10
gapC-2 3.19 +/- 0.67 maeB 1.23 +/- 0.01 mdh 0.65+/- 0.10
glk 3.81 +/- 0.14 pckA 5.56 +/- 1.77 sdhA 1.04+/- 0.01
gpmA 0.90 +/- 0.09 poxB 7.33 +/- 0.56 sdhB 1.37+/- 0.42
lpdA 0.56 +/- 0.05 ppc 1.11 +/- 0.14 sdhC 0.92+/- 0.05
pfkA 1.91 +/- 0.22 pta 2.10 +/- 0.23 sdhD 1.13+/- 0.21
pfkB 2.15 +/- 0.01 ppsA 1.26 +/- 0.22 sucA 0.81+/- 0.13
pgi 1.58 +/- 0.37 sfcA 2.05 +/- 0.34 sucB 0.80+/- 0.23
pgk 1.08 +/- 0.13   sucC 0.79+/- 0.07
pykA 1.77 +/- 0.10   sucD 1.36+/- 0.13
pykF 0.98 +/- 0.09   
tpi 1.36 +/- 0.10   
Pentose phosphate Entner-Doudoroff Glycerol uptake mal/lam and mgl/gal regulons
eda 1.83+/-0.29 glpF 7.16 +/- 0.15 galP 5.05+/-0.26
edd 4.45+/-0.29 glpK 5.60 +/- 0.87 galR 0.38+/-0.13
gnd 0.80+/-0.00 glpD 1.26 +/- 0.25 galS 0.57+/-0.01
talA 1.64+/-0.47 glpR 2.40 +/- 0.06 galT 0.49+/-0.00
talB 0.60+/-0.01   lamB 1.36+/-0.30
tktA 1.22+/-0.06   mglB 5.05+/-0.58
tktB 0.70+/-0.11   mglC 2.28+/-0.12
zwf 1.34+/-0.04   malE 1.77+/-0.20
   pgm 2.37+/-0.62
Aromatic pathway Respiration, transhydrogenases Transporters/ porins
aroA 0.81 +/- 0.03 frdA 1.58+/-0.15 actP 13.49+/-1.31
aroB 1.98 +/- 0.59 frdB 1.73+/-0.11 aroP 1.61+/-0.00
aroC 1.34 +/- 0.02 frdC 4.68+/-0.21 shiA 0.75+/-0.02
aroD 3.29 +/- 0.07 frdD 2.01+/-0.34 ompC 0.17 +/- 0.01
aroE 5.47 +/- 0.16 pntA 0.69+/-0.03 ompF 1.17 +/- 0.21
aroF 1.32 +/- 0.08 udhA 4.89+/-0.88  
aroG 0.50 +/- 0.13   
aroH 1.36 +/- 0.13   
aroK 1.01 +/- 0.07   
aroL 2.48 +/- 0.09   
Regulators   
arcA 1.25+/-0.29   
arcB 4.45+/-0.56   
creB 3.19+/-0.55   
creC 6.27+/-1.69   
crp 1.71+/-0.12   
csrA 1.03+/-0.14   
csrB 0.48+/-0.10   
cyaA 1.64+/-0.24   
fadR 1.22+/-0.31   
fruR 3.00+/-0.20   
iclR 0.29+/-0.10   
ihfA 1.15+/-0.25   
mlc 1.18+/-0.15   
pdhR 0.57+/-0.19   
ptsG 0.14 +/-0.02   
rpoS 1.04+/-0.22   
  1. Transcription levels of the measured genes of strain JM101 grown on glucose were used as controls to normalize (as one) the data using the RT-qPCR value for the corresponding gene. Results presented are the averages of at least three independent measurements of the RT-qPCR expression values for each gene. Expression levels are presented as 2−ΔΔCT values (see Methods).